Rapid determination of zygosity and common aneuploidies from amniotic fluid cells using quantitative fluorescent polymerase chain reaction following genetic amniocentesis in multiple pregnancies
نویسندگان
چکیده
منابع مشابه
Prenatal detection of trisomy 21 and 18 from amniotic fluid by quantitative fluorescent polymerase chain reaction.
Prenatal diagnosis of fetal trisomies is usually performed by cytogenetic analysis on amniotic fluid. This requires lengthy laboratory procedures and high costs, and is unsuitable for large scale screening of pregnant women. An alternative method, which is both rapid and inexpensive and suitable for diagnosing trisomies even from single fetal cells, is the fluorescent polymerase chain reaction ...
متن کاملRapid Prenatal Diagnosis of Chromosomal Aneuploidies Using Quantitative Fluorescence Polymerase Chain Reaction (QF-PCR)
Introduction: Prenatal diagnosis plays an important role in determining the health of fetus, which is why rapid diagnostic tests are so important. One of these quick diagnostic tests is quantitative fluorescent-polymerase chain (QFPCR) technique. This technique employed to detect chromosomal aneuploidies (13, 18, 21, X and Y). In this method, there is no need for cell culturing. QFPCR is rapid,...
متن کاملQuantitative fluorescent polymerase chain reaction for rapid prenatal diagnosis of fetal aneuploidies in chorionic villus sampling in a single institution
OBJECTIVE To validate quantitative fluorescent polymerase chain reaction (QF-PCR) via chorionic villus sampling (CVS) for the diagnosis of fetal aneuploidies. METHODS We retrospectively reviewed the medical records of consecutive pregnant women who had undergone CVS at Cheil General Hospital between December 2009 and June 2014. Only cases with reported QF-PCR before long-term culture (LTC) fo...
متن کاملRapid Detection of Fetal Aneuploidies by Quantitative Fluorescent-Polymerase Chain Reaction for Prenatal Diagnosis in the Turkish Population
Prenatal diagnosis is testing for diseases or conditions in a fetus or embryo before it is born. It employs a variety of techniques to determine the health and condition of an unborn fetus. The main goal of this process is to perform prenatal diagnosis at the earliest possible stage of gestation. In this regard, quantitative fluorescent-polymerase chain reaction (QF-PCR), a novel technique that...
متن کاملRapid prenatal diagnosis of congenital Toxoplasma infection by using polymerase chain reaction and amniotic fluid.
Infection of pregnant women with Toxoplasma gondii places the developing fetus at risk for congenital infection. We report a prospective study of 43 documented cases of acute maternal Toxoplasma infections acquired during gestation in which the polymerase chain reaction (PCR) was evaluated for diagnosis of fetal infection and compared with the current standard methods. On the basis of direct ly...
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ژورنال
عنوان ژورنال: Human Reproduction
سال: 2000
ISSN: 1460-2350
DOI: 10.1093/humrep/15.4.929